FastQCFastQC Report
Sun 21 May 2023
EGAF00007932224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007932224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483231
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT78611.6267582170845827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28080.5810885477132055No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26620.5508752542779747No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17360.35924847536685356No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15550.3217922691218072No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC14820.3066856224041918No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13500.27936949409288725No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13190.27295434274705055No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12670.26219344371532455No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12490.25846851712741936No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12470.2580546363954299No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12000.24832843919367756No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11910.24646597589972497No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11900.24625903553373024No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11640.24087858601786724No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10870.22494417783627293No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA10170.21045835221664175No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9750.201766856844863No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9580.1982488706229526No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9580.1982488706229526No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8730.18065893951340042No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8520.17631319182751107No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8310.1719674441416217No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8310.1719674441416217No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8230.17031192121366387No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8020.16596617352777449No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8020.16596617352777449No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA7770.1607926643779062No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7740.16017184327992204No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7730.1599649029139273No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7710.1595510221819378No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7310.15127340754214857No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6990.14465131583031718No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6840.14154721034039622No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG6790.14051250851042255No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6650.13761534338649634No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6230.1289238480147176No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6170.1276822058187492No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6160.12747526545275448No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6110.12644056362278083No Hit
CTGGCATACCGTTCTCTGACCCGCAAAAAAC6080.12581974252479664No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5860.12126705447291254No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5820.12043929300893362No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5820.12043929300893362No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5500.11381720129710221No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5360.11092003617317599No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG5340.11050615544118653No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5340.11050615544118653No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT5300.10967839397720759No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5270.1090575728792234No Hit
ACGGCAGCGACTTCCAAAGCCGTTGCTGCCA5160.10678122885328135No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5130.10616040775529714No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5030.10409100409534983No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5020.10388406372935512No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5020.10388406372935512No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4920.1018146600694078No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4840.10015913714144994No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA4840.10015913714144994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)