FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007932814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007932814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3997
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGAGGGTTCAGTAATCCCTAATCATGGAGACCAGTTACAAAGGCCTT70.17513134851138354No Hit
AGGTAACAAAGAGCAGCCTGAAAAATCAAGCTGCAGACATAGACAAGCAA60.15011258443832876No Hit
CCCTTAGGAGTTTAAGACCACTGTGGCCAACATGGTGAAACCCTGTCTCT60.15011258443832876No Hit
GACTCGAGGCGGGCAGATCACCTGAGTTCGGGAGTTTGAGACCAGCCTGA60.15011258443832876No Hit
CCGTTACAGATAGTGTCCCTGAATAAGTGGCATTATCTGCTGTGAGTAAG50.12509382036527394No Hit
GTTGTATGTTCTTAGTTAATAGTCTCTATAAATTCACTCTATTTATCTTT50.12509382036527394No Hit
CCCTAACCATAATAATAATTTATATATCAGTTCTGCTTTTATCTCTAAAA50.12509382036527394No Hit
GCACTGGGTGTGGTGGTGCGCACTTGTAATCCCAGCTACTCAGGAGGCTG50.12509382036527394No Hit
ATGTGAGACAGAGTGCCACCCTCTCCCCTTAGAAACTATGTCTGCAGTTC50.12509382036527394No Hit
TTTGTTTTCAGCCGCTTTGAGGTCAATGGTAGAAAAGGAAACTATCTTCG50.12509382036527394No Hit
CTTCTCAGTCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCGATT50.12509382036527394No Hit
TCCTGATAGGTGCTCAATAAATGTGAGACATGTTAACTATCATGACTACT50.12509382036527394No Hit
CGGTCCGTCATCTCATATCTGAAGGCCCTCCTCCCCCTTCTTAAATATCT50.12509382036527394No Hit
ATTCTGGGTGACAGAGTGAAACCCTGTCTCAAAAAAATAAAATAAAATAA50.12509382036527394No Hit
ATTCTGAGACAGGACCCAAGGGACAAAAAGTATACATAATCAACAGATGT40.10007505629221916No Hit
GGAGTAGTCACTGACTAAACGCTGCTAATGCCATCAGGAGATCCGGATAT40.10007505629221916No Hit
TTAGTTAGCAACATAGTGAGACCCTGTCTCTACAAAAAAAAAACAAAACT40.10007505629221916No Hit
AAGTCAGTGGCTCATGCCTGTAATCTCAGCACTTTGGGAGGCTGAGGCGG40.10007505629221916No Hit
ATACTGCCGTGGCTCACGCCTGTAATCCCAGCACTTCGGGAGGCCGAGAT40.10007505629221916No Hit
ATACTCTTGAGAACTTGTTAAAACAGATAGCTGAGCCTTAAATCTAGATT40.10007505629221916No Hit
GATCTTTGTTTTGGTCTGTACATTTCCCTAGGATGGTCTTAGAGAAGACT40.10007505629221916No Hit
CTACTTGGTCTCAAACTCCTGGCCTCAAGTGATCCTCCTGCCTCCGTCTC40.10007505629221916No Hit
GAGTCAATCCTCCCATCATGGCCACTTTTCTCATGGGCCCATTGGGCCAT40.10007505629221916No Hit
GCCTGCTCTCCGTCCAGTGCTCCTGTGCCATCCTTGGGGGTCTGGGATGG40.10007505629221916No Hit
TCCTTGACTTGGCTGGCTGGCTGGCTTGGCTGGCTTGGCTGGCTGGGTGG40.10007505629221916No Hit
GCCTACCCACACCCCCAGGAGAGCATCCGGCAGCCTGCAGCAGAACCCAC40.10007505629221916No Hit
TCACTCACGACCGTACCCCACACGGTTTGGAGCCCTTTCTGCCCTGCAGG40.10007505629221916No Hit
GACTAAGAACAAAGTAGGGGAGTCCACACTACCTGATTTTAAGACTTCCT40.10007505629221916No Hit
GTTGTTGGTGCCCCTCACACCTTTCTCTGGGACACATGGTCAGGCACGCA40.10007505629221916No Hit
CCAGTACTAGACCTCACCCACATACAAAACACCCCACTGCTGACAGCACC40.10007505629221916No Hit
ACCTTAATCCACTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTT40.10007505629221916No Hit
CCGTCAGGGTTTTTTCCCCCTCTTTTTGCAGTGATCAGTTTAGACAGAAA40.10007505629221916No Hit
GTCTTGGCTAGGGCAATCAGGCAGGAGAAAGAAATAAAGTGTATTCAATT40.10007505629221916No Hit
AATGTAGCTATTTTTGTGCAATGCCGTTACGTATGAAGAGAGAACTTGTT40.10007505629221916No Hit
CGTGTCATTCAAAGCAGTGTGTAGAGGGAAATTTATAGAACTAAATGCCC40.10007505629221916No Hit
AGGTAAAAGCTAGAGGTGGTGGAGTCGGGATCCGCTTATCTGGTTTCAGG40.10007505629221916No Hit
TCACTTAGGACTGAATAAGCCAGACTTTGGTATTGCTCTGTGAATTATTG40.10007505629221916No Hit
CTTCTTTCGATGACGATTCCATTCAATTCCGTTCAATGATTACATTTGAT40.10007505629221916No Hit
CCGTTTAAAAATGATTTTTAATGCCCAATGGGTTCTGAGAAAACAGTCAA40.10007505629221916No Hit
CGCTCTCTGTCACCCATGCTGGAGTGCAGTGGCACAATCACGGCTCACTG40.10007505629221916No Hit
CTGTTCTTGCCATACTTGCTTTGCTTTTGTCTTTTCCTGAAAATTCATGA40.10007505629221916No Hit
CCAGTGCTTGTAGTCGCTGCCTCTGGAGTGTGGCTCTTACTGATTGAATG40.10007505629221916No Hit
CAGTTCTAAGAGTGAAATTGCTGGCTCAAATTGGCAAGGCCATTTTTAGT40.10007505629221916No Hit
GGGTTGCAATCCTGAATGCATAAAGAATTAAAACTGCAGAGAAAGAGGAG40.10007505629221916No Hit
ATGTTCCACTTTCAGTTTTGTTTTATTTTTAACAATATGGCAGTTTTCAA40.10007505629221916No Hit
TAGTTATACGAAGATATATCCTTTTCTGCCTTTGGTCTCAAAGCGCTTGA40.10007505629221916No Hit
GCCTGGGCACAAGTGATTCTCCCGCCTCAGCCTCCCGAATAGCTGGGAAT40.10007505629221916No Hit
GTGTTTCTCAAGCACTTCCTGGGTGCCAAAGTCACCTGGTTGGAAGGTGC40.10007505629221916No Hit
GTGTCCTTCTCAGTAGTATACATTTACATGTTTTCACTTGGGATCTTATT40.10007505629221916No Hit
TTTGTTTTTATATTTTTATATATAAAAAGCAAGAATCTGAGTGTTAATAC40.10007505629221916No Hit
ATACTGGGAGGAAGCGGGATTCCGGTCATAAAAACCACCCCGTGGCCCTC40.10007505629221916No Hit
AAGTAGCACACCTGGCTGTATATAATTGTCCTTGATTCTAATAGCAAAAT40.10007505629221916No Hit
TGTCTAATATCCCCTATTCCCAGAGCTCCAGGTTCCCACCATTCAGGAGA40.10007505629221916No Hit
ACAGTCAAACACTACAAATACTCACTAAACAGAACCATTTCAGGTTTTAC40.10007505629221916No Hit
AGTCTGCCAATAAAGACTTATTGAGCAACTACTCTACAATAGGCAACAGG40.10007505629221916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)