FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007933508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007933508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTTTTATTGTGATCATAGATTCTGCAGATCAGGGTTTCTAGAAGGGCT60.1957585644371941No Hit
AATGTCTGCCCTGGCCCCTTCGTTGATTCATCCATTTGTTCCTGGTTTGC50.1631321370309951No Hit
GCTCTGATCTGAGAACCGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCC50.1631321370309951No Hit
AGACTAACCCCAGTACTTTGGGAGGCGAAGGCAGGAGAATTGCTTGAGTC50.1631321370309951No Hit
AATGTTCTTTTCCACAAGTTCAGCCCGTGGCTCACTGATTATTTCCTCCT50.1631321370309951No Hit
GAACTGGACGCTGCGGCGCACCGACGCGAGGGCGTCGATTCCCGTTCGCG50.1631321370309951No Hit
AAAGTTCCCAGGATCTGGAACTGGGGAGGGGTGGGCCAGGCAGACAGGCC50.1631321370309951No Hit
ACGTAGCATTCAGAAAAGAATACTCTTCAAAGTATTTGCGGATACCAACT50.1631321370309951No Hit
AGGTAGCATTCCAAAGTCATGAGCACCATCAGATGCATAGGGGCATAATT50.1631321370309951No Hit
CCCTAAATGCCAGGCATGGTGGCTCATGCCTGTAATCCCAGCATTTTGGG40.13050570962479607No Hit
CTGTCTTCAAAGCGGCTGAAATCTCCACTTGCAAATTCCACAAAAAGAGT40.13050570962479607No Hit
ATTCTTGGCTAACTAAAAAAAAATTTTTAAGAGATGAGGGGTCTCACTAT40.13050570962479607No Hit
TTAGTCTGGAGGGGGAAGAGAGCCCAGGGATCATGGCTCCTCCACCAGCT40.13050570962479607No Hit
CTCTGATGGAGGGAGTAGGCAAAAGGAAGGGGAAAAGAGGGAGAAGAGAC40.13050570962479607No Hit
TAAGTTCTTTCTTTTTATTCTCTTTAGACGGAGTTTCACTCTTGTCGCCC40.13050570962479607No Hit
CAACTTGATGGAGTCTTTTAGCCTGATTTGGACTGGGTGGGGATTTTTTG40.13050570962479607No Hit
GACTCTTCCTTGCATTGGGTAGAACATGTTCCTTTAGCTCAGAGGAGTTT40.13050570962479607No Hit
CCCTTTTTTGAGATGGAGTCTCGCTCTGTACCCCAGGCTGGAGTGCAGTG40.13050570962479607No Hit
AAAGTGCCCTAATTTCTGGATCTTTTCCAATCACAAATACAATGTGGATC40.13050570962479607No Hit
ATTCTAAGTCATAGCTGCAAGCTAAGGGACTGAAACTTCAACCAATCATA40.13050570962479607No Hit
CTACTCTGTGGGTCTGTGTCTACTAAAAACTTGATAGTATCTGATATGGT40.13050570962479607No Hit
CATCTTGCTTGCTTAATAAGTACTCCTGAATGAACAGTGGAAGAACTTTC40.13050570962479607No Hit
GATCTTCCATTTGTGCAAGCAGCTGGGCTCTAGTTTCATTCCATCTGTCA40.13050570962479607No Hit
CGTGTGTATTCTTTTTTTCTTTTCTTTTCTTTTCTTTTTTTTAAGACAGA40.13050570962479607No Hit
TAAGTAATGATTCCGTCTATTTTTTCTACGAAGATATTTCCTTTTCTGCC40.13050570962479607No Hit
CTTCTTTTTCCAACTTGGTTCCATTCTTCCTGTCACTTTCAGGTACACCA40.13050570962479607No Hit
ATACTCCAGGCACAGTGGCTCACACCTGTAATCCCAACACTTTGGGAGGC40.13050570962479607No Hit
CCGTTTGTTGTGGAATGTGCAAGTGGAGATTTGGAGCGCTTTGAGGCCTA40.13050570962479607No Hit
GAACTTAGCCCAGGCACTTACTATAAAAAATTAAACGGGGGAGGGTTCCA40.13050570962479607No Hit
GATCTCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGG40.13050570962479607No Hit
TGCTGAGGAGGAGGAGGCGGCAGCGTGGCCAAGCCCTGGGGGCACAAAGA40.13050570962479607No Hit
AGCTTAGGGTTGGTATGCATAGTTTCAGATTGCCCTCCAACTCTGACCAA40.13050570962479607No Hit
ACGTAACTCTGTGAGTTGCATACACACAACACAAAGAAGTTACTGAGAAA40.13050570962479607No Hit
AGACTGTGTGATGGCACATGCTTGTAATCCCAGCTACTCAGGAGGCTGAG40.13050570962479607No Hit
AGGTCAGCATCTGAGAAAAAAATTTGTACACACAAAAGTTCTGATTTTTT40.13050570962479607No Hit
AGTCTATCAGCACCCTCTGTTTAGCTCAAGGTTTGTGAGTGCACCAATCG40.13050570962479607No Hit
TCGTACCATGTTGCCCAGGTTTGTCTTGAACCCCTGAGCTCAAGTAATCC40.13050570962479607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)