FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550934
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA81931.487110978810529No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA72511.3161286106865795No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT37350.6779396443131119No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26550.48190890378883855No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA24700.4483295639768103No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC24090.4372574573360874No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT22690.41184606504590393No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT17940.3256288412042096No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15180.27553209640356197No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14540.26391545992804943No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT14460.2624633803686104No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG13200.2395931273074452No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13100.23777802785814633No Hit
GTCGGAAATCTGTTTCTCTTCCTTAACTTTATCCAGTTCCGCGG11180.20292811843160888No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA10880.1974828200837124No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT10320.18731826316763894No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10280.18659222338791942No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9920.18005786537044363No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9450.17152689795873916No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8870.16099932115280596No Hit
TGGAATGGTTTTGCAGATGGATTTGCACAGCTGGTTGTTACCGC8100.14702305539320498No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT7830.14212228688009818No Hit
TGGGATAACATCGGTCGGAATGTAGGACAGAGATTCCTCCGCGT7240.13141320012923507No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7210.13086867029444543No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7120.12923508079007648No Hit
GCCTTGACGAGATGCGTCGGAAATCTGTTTTTCTTCTTTGACCT7070.12832753106542708No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT6840.12415280233203976No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6810.12360827249725012No Hit
TTCTTCAGTGTGGTCCTCTTCAGATTTTTTCTTGTCCTCGCTCT6730.12215619293781106No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6360.1154403249754054No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG6360.1154403249754054No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6080.11035804651736869No Hit
TCAACCAATGTGTGGGGTAGTTGCGACGGTGTCTACCAGGATGC5910.10727237745356069No Hit
GTCAACCTGGTTGTCGTTCGCGATTTTCTCGGTGATAGCTTCCA5780.1049127481694722No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5770.10473123822454232No Hit
GGTCGGTTTTTTCTTCGGTTTGTTGGACGGAATGGTCTTGCAGA5700.10346066861003314No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA5540.10055650949115501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)