FastQCFastQC Report
Sun 21 May 2023
EGAF00007934657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550934
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT191003.4668399481607595No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38350.6960906388061002No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT37260.676306054808743No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26700.4846315529627868No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA24160.4385280269505966No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17020.30892992627066035No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15030.2728094472296137No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14810.26881622844115627No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA14570.2644599897628391No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14140.25665506213085415No Hit
AAACAAGATTTCGGTGCGCTGAAACAGGAGC11720.21272965545782252No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA11220.2036541582113284No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11160.2025650985417491No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10860.1971198001938526No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC10640.1931265814053952No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT10420.18913336261693778No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9880.1793318255907241No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9670.1755201167471966No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG8970.1628144206021048No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8020.14557097583376594No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7770.14103322721051886No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7730.14030718743079934No Hit
AACCTGACCGCGGAACTGGATAAAGTTAAAG7610.13812906809164074No Hit
CCGAAAGACCAGTTTCTGGAAGCAATCACCG7500.13613245869741203No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7230.1312316901843052No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT7170.1301426305147259No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7120.12923508079007648No Hit
ACCACCACTTCTACTCAGACGAATAAACCGA6940.1259679017813386No Hit
ACCACCAACGAACAGCCGAAACCAGAATCTT6880.12487884211175931No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6750.12251921282767082No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6370.1156218349203353No Hit
GTGCGCCAGGAAAACCTGCAGATTCTGATGA6370.1156218349203353No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6360.1154403249754054No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC6270.11380673547103645No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6250.1134437155811767No Hit
CCGAGCACTAAACCGCGTCCGAAAAACCCTC6190.1123546559115974No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT6050.10981351668257905No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5880.10672784761877105No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5840.1060018078390515No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5840.1060018078390515No Hit
ACCGCAGAGCTGGATAAAGTTAAAGAAGAAA5740.10418670838975266No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5670.1029161387752435No Hit
GTTGGCAAAAGCATTGAAGATGCTCGTGAAG5600.1016455691607343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)