FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531592
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA78891.484032867311773No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA71551.3459570497674909No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT35460.6670529278092974No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC25170.473483423377327No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC23570.4433851525229875No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT23070.4339794428810065No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA22900.43078150160273293No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT17300.32543755361254495No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14740.2772803202456019No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14430.27144878026757363No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13460.2532017035621304No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12640.2377763397492814No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG12630.2375882255564418No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT11170.21012355340185707No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA11050.2078661830877816No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9820.18472813736850818No Hit
GTCGGAAATCTGTTTCTCTTCCTTAACTTTATCCAGTTCCGCGG9700.18247076705443271No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT9490.17852036900480067No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8890.16723351743442338No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8540.16064952068503663No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT7980.15011512588601786No Hit
TGGAATGGTTTTGCAGATGGATTTGCACAGCTGGTTGTTACCGC7650.14390735752231035No Hit
GCCTTGACGAGATGCGTCGGAAATCTGTTTTTCTTCTTTGACCT7180.13506599045884812No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT7020.1320561633734142No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6860.12904633628798026No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6750.12697708016674442No Hit
TGGGATAACATCGGTCGGAATGTAGGACAGAGATTCCTCCGCGT6670.12547216662402744No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6660.12528405243118781No Hit
TTCTTCAGTGTGGTCCTCTTCAGATTTTTTCTTGTCCTCGCTCT6570.12359102469563124No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6360.11964062664599917No Hit
TCAACCAATGTGTGGGGTAGTTGCGACGGTGTCTACCAGGATGC5980.11249228731809358No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG5970.11230417312525397No Hit
GTCAACCTGGTTGTCGTTCGCGATTTTCTCGGTGATAGCTTCCA5760.1083537750756219No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5600.10534394799018797No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA5510.10365092025463137No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT5500.10346280606179174No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5390.10139354994055591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)