FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007934659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531592
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT181213.408817288446779No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46730.8790576231395506No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC36310.6830426342006651No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT35220.6625381871811464No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24130.45391954732200634No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA23710.44601875122274226No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16160.3039925356288281No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15200.28593357311622447No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA13840.26035004289003594No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13640.25658775903324355No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13400.2520730184050926No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11500.21633132176556458No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA10960.206173155352225No Hit
AAACAAGATTTCGGTGCGCTGAAACAGGAGC10310.19394573281764962No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT10040.18886664961097985No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9610.18077773931887614No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC9590.18040151093319687No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9380.17645111288356483No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG8700.1636593477704706No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7890.14842209815046126No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7680.14447170010082921No Hit
AACCTGACCGCGGAACTGGATAAAGTTAAAG7570.14240244397959337No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7270.13675901819440475No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7070.1329967343376123No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT7050.13262050595193306No Hit
ACCACCACTTCTACTCAGACGAATAAACCGA6840.12867010790230102No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6830.1284819937094614No Hit
CCGAAAGACCAGTTTCTGGAAGCAATCACCG6770.12735330855242366No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6750.12697708016674442No Hit
ACCACCAACGAACAGCCGAAACCAGAATCTT6730.1266008517810652No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6500.12227422534575388No Hit
GTGCGCCAGGAAAACCTGCAGATTCTGATGA6270.11794759891044258No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT5980.11249228731809358No Hit
CCGAGCACTAAACCGCGTCCGAAAAACCCTC5890.11079925958253699No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC5810.10929434603982001No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5790.10891811765414076No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5580.10496771960450872No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5540.10421526283315025No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5530.10402714864031061No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5510.10365092025463137No Hit
CGTTACATTTTTGCGGGTTATAAAACCGAGA5420.10195789251907478No Hit
GTCGTTGTATCTGCAAAAAAATTCGCATCTA5390.10139354994055591No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5380.1012054357477163No Hit
GTTGGCAAAAGCATTGAAGATGCTCGTGAAG5350.10064109316919743No Hit
ACCGCAGAGCTGGATAAAGTTAAAGAAGAAA5350.10064109316919743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)