FastQCFastQC Report
Sun 21 May 2023
EGAF00007934670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423562
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA96302.2735750610300265No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA95632.257756833710295No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC46951.1084563771065392No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA39890.9417747578866847No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA33060.7805232764034545No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA28740.6785311241329486No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC27140.6407562529216502No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT26090.6159664936892356No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT23200.5477356325638277No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA22920.5411250301018504No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA17100.40371893607075227No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG16080.37963745567354956No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT15220.35933346239747666No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG14990.3539033246608525No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13870.3274609148129436No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11680.2757565598424788No Hit
TTCAGACGGTGGGGTGTCCTCGGACTGGCCCCAGTCGCTCGGGC11460.2705625150509252No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT10060.2375095027410391No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10060.2375095027410391No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9190.21696941651989554No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8560.2020955609804468No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC8170.19288793612269278No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8060.190290913726916No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG7910.18674951955085678No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7220.17045910634098432No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7120.16809817689027817No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA6420.1515716707353351No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT6320.14921074128462894No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC6260.14779418361420524No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT6200.14637762594378154No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA6050.1428362317677223No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5880.13882265170152186No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5870.13858655875645123No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT5500.12985111978883848No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5400.12749019033813233No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5280.12465707499728493No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG5250.12394879616207308No Hit
GTCAATCAGGGTACAGTTTTTACCATCCAGGATGCGGTGCGGAG5100.12040740198601385No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5070.11969912315080201No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5050.11922693726066078No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT5030.11875475137051955No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5010.11828256548037833No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG4820.11379679952403662No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG4710.11119977712825985No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4640.10954712651276555No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4560.10765838295220063No Hit
CGGGCCTTCACCTGGATCGTCCGCCGGACCCTGTTCGATAGCAC4530.10695010411698878No Hit
CAGTGCATCGATCAGAGTACAGTTTTTGCCATCCAGGATACGAT4450.10506136055642384No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTACCAAAGTGTCGGCACG4260.10057559460008217No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG4260.10057559460008217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)