FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423562
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT233655.516311661574929No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC64621.5256326110463168No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA40450.9549959628106394No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG40370.9531072192500745No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT32490.7670659785344295No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC29630.6995433962442335No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG25880.6110085418427527No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC19030.4492848744693811No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA16880.3985248912791988No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC16370.3864841510805974No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC15610.36854108725523066No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT14580.34422351391295725No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13960.3295857513185791No Hit
GGTACCGGCTACGAAAAACGCGATGACTGGC12100.2856724635354446No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11240.2653684702593717No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10750.25379991595091156No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC10560.24931414999456986No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9260.21862206713538987No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9180.21673332357482494No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT8740.20634523399171786No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG8210.19383230790297523No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8130.19194356434241033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7880.18604124071564493No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7830.18486077599029185No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7780.18368031126493878No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA6980.16479287565928954No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6740.1591266449775948No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6670.1574739943621005No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC6610.15605743669167677No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6290.1485024624494171No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6290.1485024624494171No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5930.14000311642687494No Hit
ATAACCCAGGGTCGTCGTGGTAACGTTTACT5920.1397670234818043No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5880.13882265170152186No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5880.13882265170152186No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5840.1378782799212394No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG5510.13008721273390908No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5420.12796237622827356No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC5250.12394879616207308No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5200.12276833143672No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5180.12229614554657878No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5130.1211156808212257No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC5130.1211156808212257No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5080.11993521609587263No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4890.11544945013953092No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4780.11285242774375416No Hit
CTGGGTCACCACGCGGTTCCGAACGGCACTC4670.1102554053479774No Hit
CACCACGCGGTTCCGAACGGTACGCTGGTAA4670.1102554053479774No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC4500.10624182528177692No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4460.10529745350149446No Hit
CTGGGCCACCACGCAGTACCAAACGGTACGC4430.10458917466628262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)