FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399446
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA90672.26989380291704No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA88132.2063057334408156No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC44021.1020263064344118No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA35770.8954902540017925No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA30850.772319662732885No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA26320.6589125939426105No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC24610.6161033030747586No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT23970.6000811123405917No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA22770.570039504714029No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT20910.5234750128928567No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA16090.4028078889261627No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG15080.37752286917380573No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG14570.36475518593251655No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT13700.3429750204032585No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13600.34047155310104493No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11050.276633136894599No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10590.26511718730441663No Hit
TTCAGACGGTGGGGTGTCCTCGGACTGGCCCCAGTCGCTCGGGC10280.2573564386675546No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9170.229567951612984No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8780.21980442913435105No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8330.20853882627439005No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8180.20478362532106967No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG7370.18450554017313978No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC7290.18250276633136894No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6960.1742413242340642No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6780.16973508309007976No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA6350.15897017369056143No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5870.1469535306399363No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC5820.1457017969888295No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5790.14495075679816546No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5750.14394936987728002No Hit
GTCAATCAGGGTACAGTTTTTACCATCCAGGATGCGGTGCGGAG5580.139693475463517No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5550.1389424352728529No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5250.1314320333662122No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT5060.12667544549200643No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5030.12592440530134236No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG5030.12592440530134236No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4970.1244223249200142No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT4810.12041677723647251No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4790.1199160837760298No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC4690.11741261647381623No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT4590.11490914917160266No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG4570.11440845571115996No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4520.11315672206005317No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG4330.1084001341858474No Hit
CGGGCCTTCACCTGGATCGTCCGCCGGACCCTGTTCGATAGCAC4300.10764909399518333No Hit
CAGTGCATCGATCAGAGTACAGTTTTTGCCATCCAGGATACGAT4190.10489527996274842No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG4100.1026421593907562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)