FastQCFastQC Report
Wed 31 May 2023
EGAF00007934673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399446
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT212285.314360389138958No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC61081.529117828192046No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG36970.9255318616283552No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36860.9227780475959204No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA36320.9092593241639672No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT28460.7124867942099808No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC26780.6704285435327929No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG24190.6055887404054615No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC17670.442362672301137No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA15900.39805130105195696No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC15000.37552009533203484No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC14830.3712642009182718No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13480.3374673923383887No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13370.3347135783059538No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11690.29265532762876584No Hit
GGTACCGGCTACGAAAAACGCGATGACTGGC10750.2691227349879583No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9300.23282245910586163No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9200.23031899180364804No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8570.2145471477997026No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8540.21379610760903853No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8460.21179333376726764No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT8310.2080381328139473No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7820.19577114303310084No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7440.1862579672846893No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7020.1757434046153923No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC6810.17048612328074383No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6290.1574680933092333No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6190.1549646260070197No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA6190.1549646260070197No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5940.1487059577514858No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5810.14545145025860817No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5720.14319832968661597No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5510.13794104835196747No Hit
ATAACCCAGGGTCGTCGTGGTAACGTTTACT5300.132683767017319No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5210.1304306464453268No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5190.12992995298488408No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5050.12642509876178507No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG5040.1261747520315637No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC4900.12266989780846471No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4820.12066712396669388No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4810.12041677723647251No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC4740.118664350124923No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4740.118664350124923No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4590.11490914917160266No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4550.11390776225071723No Hit
CACCACGCGGTTCCGAACGGTACGCTGGTAA4500.11265602859961046No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4440.11115394821828231No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4200.10514562669296976No Hit
CTGGGTCACCACGCGGTTCCGAACGGCACTC4130.10339319958142026No Hit
CTGGGCCACCACGCAGTACCAAACGGTACGC4090.10239181266053483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)