FastQCFastQC Report
Wed 31 May 2023
EGAF00007934682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427407
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT55951.3090567070731176No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG37650.8808933873333847No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG27320.639203382256257No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA26620.6228255503536442No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT26340.6162744175925992No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25340.5928775148745808No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT24840.5811790635155718No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC24750.5790733422709502No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG20170.47191552782242685No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC19220.4496884702403096No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18820.44032970915310227No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG18730.4382239879084807No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18440.43143888612025544No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA18260.4272274436310121No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT16320.38183745235805683No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15860.3710748771077685No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15030.3516554478518134No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG13530.3165600937747861No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG13310.3114127751768221No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC13190.30860514685065993No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA12710.2973746335460112No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12660.2962047884101103No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12440.29105746981214625No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10880.2545583015720379No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10860.25409036351767755No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG10840.2536224254633172No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10530.24636938562073155No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC10530.24636938562073155No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9270.2168892881960286No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG9140.21384769084268626No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9010.21080609348934387No Hit
CATGCTGTCCTGCAGCGGCATCGGTGGTGGCGGGAAGCGGGTAG8930.20893434127190244No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8560.2002774872662357No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT8300.19419429255955095No Hit
GGAGATTTCGCTGTTGTCGGACAGGATTTCCTGAGCTTCTGCGG7410.17337104914051477No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7250.16962754470563188No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC7240.1693935756784517No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6890.16120465972714532No Hit
CTGAGGCTGGGTCGCCGGAACCGGTGGAACCGGACCCATGCTGG6800.15909893848252368No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG6790.1588649694553435No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6580.1539516198845597No Hit
CGGAGAACCGCCCTGACCCATAACCTGAATCGGCTGCAGGACCG6420.15020811544967677No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6380.14927223934095604No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT6140.14365698268863167No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG6010.1406153853352893No Hit
GCTCGGCTCCTGCTGCATCTGGGTGATTTCGCGGCCAGCACGCA5870.13733981895476677No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5700.13336234549270368No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5600.13102265522090187No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5560.13008677911218114No Hit
AACCAGCGGTACCAGGGACAGCTGCAGGCGTGGCGGACCGGTTT5550.12985281008500096No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5480.12821502689473968No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5360.1254073985685775No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG5090.11909023483471258No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5080.1188562658075324No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5050.11815435872599186No Hit
CAGCGCGCGCAGGCCCTCAGCGATGTTTTCGAACTTCGGGTTGG4980.1165165755357306No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4860.1137089472095684No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT4830.11300704012802784No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG4720.11043338082904586No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA4560.10668987639416296No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4520.1057540002854422No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4350.10177652682337912No Hit
CTGCCAACCCGCCGCTGCCTGACGTTCCAGGGCAGCCTGCAGTT4330.10130858876901876No Hit
CAGTGCACGCAGGCCTTCAGCGATGTTTTCGAACTTTGGGTTGG4320.10107461974183858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)