FastQCFastQC Report
Wed 31 May 2023
EGAF00007934683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427407
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA56371.3188834062146855No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA48761.140832976530567No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC42240.9882851708090883No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG32510.7606333073627713No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32000.748700886976582No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG31590.7391081568621946No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26690.6244633335439055No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26500.6200179220274821No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC25690.6010664308258873No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22330.5224528376933462No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC21530.5037353155189316No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21140.49461052345890455No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19700.4609189835449583No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18940.4431373374792645No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT18010.42137821795150754No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17630.41248739491866065No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC16590.38815461609192176No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16480.38558095679293974No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15990.37411647446111085No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15740.36826724878160627No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA14340.33551158497638084No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13490.31562421766606535No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG12980.3036917972798761No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12550.29363112911112826No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11930.279125049425957No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT11080.2592376821156415No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC10380.2428598502130288No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10080.23584077939762335No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9060.21197593862524478No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG8990.21033815543498352No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8670.20285114656521772No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC8340.19513016866827168No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8030.18787712882568608No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7920.18530346952670404No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7820.18296377925490223No Hit
CCGTTCGCCTCTGATTACTCCCAGGGTGCGT7600.17781646065693824No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7190.1682237305425508No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7120.1665859473522895No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC7050.16494816416202823No Hit
GGCCGCCCTTCCCTGAAACTGCAGGCTGCCC6960.1628424429174066No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6700.15675924821072185No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA6660.15582337210200112No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6360.1488043012865957No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC5840.13663791187322621No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5790.1354680667373253No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5790.1354680667373253No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5780.13523409771014513No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5730.1340642525742442No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5710.13359631451988385No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5520.1291509030034604No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC5470.1279810578675595No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5310.12423755343267658No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5250.12283373926959548No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5140.1202600799706135No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG5080.1188562658075324No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5030.1176864206716315No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4940.11558069942700984No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG4850.1134749781823882No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4750.1111352879105864No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4710.11019941180186567No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4660.10902956666596476No Hit
CCGTTCGCAAGCGACTACTCTCAGGGTGCAT4610.10785972153006386No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4580.10715781444852332No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG4550.10645590736698277No Hit
GTACTGCCGACCCCGATGCAGCTGGCACTGC4490.10505209320390166No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4430.10364827904082057No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4370.10224446487773949No Hit
GGTTACCCAGGTCCGCGCGGTGTCAAAGGTG4320.10107461974183858No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4300.10060668168747822No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4300.10060668168747822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)