FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007934685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437562
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA57121.305415004045141No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA50391.1516082292338No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC42280.9662630667196878No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG32970.7534932192466439No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32370.7397808767671782No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG32240.7368098692299606No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27410.6264255122702611No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC26220.599229366352654No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25980.5937444293608677No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22890.523125865591619No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22800.5210690142196992No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC22120.5055283594096379No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19900.45479269223561464No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18470.42211160932622116No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT17880.40862780588807984No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC17510.40017186135907595No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16820.3844026675076903No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15820.3615487633752474No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14910.3407517106147243No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA14780.33778070307750674No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG13840.31629803319301036No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13290.3037283859201667No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13200.30167153454824686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13010.2973292927630827No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG12150.2776749352091818No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC11330.2589347338205786No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT11290.25802057765528086No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10610.24247992284521963No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG9210.21048445705979954No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8760.20020020020020018No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8740.19974312211755132No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8690.19860042691092922No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC8510.19448672416708945No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8150.18625931867941No Hit
CCGTTCGCCTCTGATTACTCCCAGGGTGCGT7680.17551798373716182No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7380.16866181249742893No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7370.1684332734561045No Hit
GGCCGCCCTTCCCTGAAACTGCAGGCTGCCC7180.16409103167094036No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA6980.15952025084445176No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6800.15540654810061202No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6780.15494947001796316No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC6760.1544923919353143No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6700.15312115768736773No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC6410.14649352548895928No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6120.13986589329055082No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6060.13849465904260425No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC5960.13620926862935995No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5940.1357521905467111No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5850.13369533917479123No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5810.13278118300949351No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5760.13163848780287135No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC5730.13095287067889808No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5600.1279818631416805No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5360.12249692614989419No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5200.11884030148870332No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5160.1179261453234056No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG5110.11678345011678344No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5020.11472659874486359No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG4930.11266974737294373No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4930.11266974737294373No Hit
GGTTACCCAGGTCCGCGCGGTGTCAAAGGTG4900.11198413024897044No Hit
GTACTGCCGACCCCGATGCAGCTGGCACTGC4890.111755591207646No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG4880.11152705216632157No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4720.1078704275051307No Hit
CCGTTCGCAAGCGACTACTCTCAGGGTGCAT4640.10604211517453525No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT4500.10284256859599325No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4460.10192841243069554No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4440.10147133434804667No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4400.10055717818274895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)