FastQCFastQC Report
Wed 31 May 2023
EGAF00007934686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432194
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA76371.7670305464675586No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA71501.6543496670476683No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT34260.7926995747280157No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT32960.7626204898726034No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG29650.686034512279208No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC28960.6700694595482585No Hit
TTTAGCTTCACGACCACGAGAACGAGAGCGGGCGCGACGGGTTG24300.5622475092203965No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG21730.5027834722370047No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA20110.46530030495564495No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC18930.43799775100996313No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT17690.40930693160941617No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT16540.3826985103911669No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT15720.3637255491746762No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC15680.36280003887143275No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG15680.36280003887143275No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT14630.33850539341129215No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14270.33017580068210106No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG13260.30680666552520397No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13120.30356737946385187No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11190.25891150733235535No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10730.2482681388450557No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT10670.24687987339019052No Hit
AATCAGCGGGTTGAATGCCTGTTTTACCTTTTTCGGGTGTTTCT9780.22628726914302372No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8860.20500053216842437No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA8710.20152986853126142No Hit
GATACGGTGGGCGGTCAGATCGTTCTCCCACGCGAAGTCATCCA7930.18348241761801412No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT7590.17561558004044478No Hit
AGTACCCGGACGGAAGTTGGACTGGAACAGCAGGGTGTCGGTAG7450.1723762939790927No Hit
ACGACATTTACGGGAAGCGACACGGTTTTTGTAACGCTTAATCT7320.16936838549355152No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7160.16566634428057772No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7060.16335256852246907No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6890.1594191497336844No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6610.15294057761098023No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6580.15224644488354766No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6560.15178368973192594No Hit
GATGAGCGGGTTGAACGCCTGTTTAACCTTTTTAGGGTGTTTCT6400.14808164851895214No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6380.14761889336733042No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6250.1446109848817892No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5720.13234797336381346No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT5380.12448113578624415No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5340.1235556254830007No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5340.1235556254830007No Hit
GATCAGTGGGTTGAACGCCTGTTTAACTTTTTTTGGGTGTTTCT5060.11707705336029654No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT4860.11244950184407929No Hit
TTTCGGGTGTTTCTGCTTCTGACGGGCTTCGCTGGAGGTACGTT4800.1110612363892141No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA4650.10759057275205115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)