FastQCFastQC Report
Wed 31 May 2023
EGAF00007934687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432194
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT175534.0613705882080735No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA68421.5830853736979227No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT33970.7859896250295006No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA30100.6964465031906968No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA29710.6874227777340731No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24750.5726595001318853No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC23850.5518355183089076No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA21920.5071796461774111No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC20960.4849673988995683No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17260.3993576958495491No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG17200.3979694303946839No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG16570.3833926431185995No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG16260.3762199382684628No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14290.3306385558337228No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12860.29755156249276943No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12710.2940808988556065No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11660.2697862533954659No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11070.256134976422625No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10900.25220155763384033No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10450.24178956672235152No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC9440.2184204315654544No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA9110.2107849715636959No Hit
ACCGATGGTGACGGTACTACTGATACCCTGC8270.19134925519558346No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT7750.1793176212534186No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7440.17214491640328186No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7170.16589772185638857No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG7120.16474083397733424No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7070.16358394609827992No Hit
AACCCACTGGGCCGTTTTCGTCCGGCCGAAA6990.16173292549179302No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6820.15779950670300838No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6650.15386608791422368No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG6620.1531719551867911No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6510.1506268018528716No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6330.1464620054882761No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6320.14623062791246524No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT6210.14368547457854575No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG5720.13234797336381346No Hit
GCACGCCCGGAAACCCCGTCCCCGGCAATTC5430.12563802366529847No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5340.1235556254830007No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5290.12239873760394639No Hit
CCGCCTCGTACCCCAACCTGGGAATCTCCGG4660.10782195032786202No Hit
GACGCCGCGTCTTCTTCTTCCGCGGGTCAGT4510.10435128669069908No Hit
CAGGGCCAGCAGCTGGCCGATATCGGTGTGG4400.1018061333567796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)