FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413181
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26020.6297482217236514No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA19370.4688018084084215No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19150.4634772654115267No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18490.44750363642084223No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16840.4075695639441311No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16380.39643642858698724No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG15640.3785266021428865No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14620.3538400846118287No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14010.3390765790295294No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13460.32576522153729237No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12850.3110017159549931No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12800.3097915925466079No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11770.28486305033387305No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10140.24541302722051594No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC10100.24444492849380778No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10080.24396087913045372No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9380.2270191514130611No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9120.22072650968945812No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8530.20644705347051293No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8490.20547895474380476No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8270.20015441174690995No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7720.18684305425467287No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC7670.1856329308462877No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7580.18345470871119438No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7550.18272863466616326No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7080.17135347462734252No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT7060.17086942526398843No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6750.16336666013200027No Hit
CTGCCGGTTACCGTCACCATCGCCCCGATGT6660.16118843799690694No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6520.1578000924534284No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6030.14594088305125355No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5990.1449727843245454No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5730.13868014260094244No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5710.13819609323758839No Hit
TGTGAACGTTTCGGCGCCGCCGTCACTGACA5650.13674394514752614No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA5640.1365019204658491No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5360.12972522937889205No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5080.12294853829193501No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4830.11689792125000907No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4820.11665589656833204No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4730.11447767443323871No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4510.10915313143634386No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC4450.10770098334628167No Hit
CGTCGCGTTGCAGCGTCCGCTAGCGTCCCGC4410.1067328846195735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)