FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380540
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT86152.263888158932044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57701.5162663583328955No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA56341.4805276712040785No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25620.6732537972355074No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21380.5618331844221369No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17230.45277763178640884No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16110.42334577179797134No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC12700.3337362695117465No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11720.30798339202186364No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10590.2782887475692437No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10230.2688285068586745No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC10210.2683029379303095No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT8370.21995059652073368No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8170.21469490723708415No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6950.1826352026068219No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6910.18158406475009198No Hit
TCTAACAGCGTAAACGGTTCTTCCCAGGCCG6180.16240079886477113No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5730.15057549797655964No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5610.1474220844063699No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5610.1474220844063699No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5490.14426867083618017No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5480.1440058863719977No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC5450.14321753297945025No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5120.1345456456614285No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA4580.12035528459557472No Hit
ACCACCCTGCCGCCGACTCCGCTGCTGACCG4390.11536237977610762No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4360.11457402638356022No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4360.11457402638356022No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4180.10984390602827561No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG3910.10274872549534872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)