FastQCFastQC Report
Sun 21 May 2023
EGAF00007934694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395279
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA39961.0109315192560193No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA37410.9464201235076997No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT29320.7417545581728349No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC24690.624622102363141No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18630.47131266776125214No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17650.44652005292464314No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT17240.43614763243177607No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC13740.34760257944388645No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC12940.3273637101895117No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG12720.32179802114455863No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12020.30408901054698073No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG10190.2577925971275985No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA9850.2491910776944892No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG9180.23224102469395036No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8980.22718130738035666No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG8700.2200977031413255No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7770.19657001763311482No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7580.19176328618520083No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7340.1856916254088884No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6940.17557219078170103No Hit
GGATGCGGACGCAGTGGTAGCACGAGACTGACGCGGGGCGGTAG5900.14926166075101382No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5850.14799673142261544No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5650.14293701410902174No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5510.13939521198950616No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5470.13838326852678742No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT5320.13458848054159214No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG5260.13307056534751402No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4840.12244515898896728No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT4780.12092724379488917No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4700.11890335686945169No Hit
CGGTTTGTCAACCGCGGTGGCGTCATCGTCGCTGCAATAGCTTG4610.11662648407833455No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4540.11485558301857675No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4440.1123257243617799No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT4330.10954287983930339No Hit
GTTGTAGAAAACGGTAGGGCTACGCGGTTCCGGGGAATCTGGGT4160.10524212012274874No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4130.10448316252570969No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG4100.10372420492867063No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA4060.10271226146595189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)