FastQCFastQC Report
Sun 21 May 2023
EGAF00007934695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395279
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT90752.2958467310431367No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA59001.4926166075101384No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53121.343860918490484No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27220.6886275263801012No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22280.563652508734337No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17870.45208574196959617No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17440.4412073497453697No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC13550.34279584799597246No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13020.3293875971149492No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10570.2674060600234265No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10270.259816484053036No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC10150.2567806536648798No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9110.2304701236341926No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT8860.22414547699220044No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7480.18923342752840397No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6930.17531920491602135No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6130.15508033566164658No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5930.1500206183480529No Hit
TCTAACAGCGTAAACGGTTCTTCCCAGGCCG5890.14900867488533415No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5560.14066014131790455No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5550.14040715545222487No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5330.13484146640727182No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5180.13104667842207654No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC5130.12978174909367815No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4860.12295113072032665No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4830.1221921731232876No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA4670.11814439927241266No Hit
ACCACCCTGCCGCCGACTCCGCTGCTGACCG4570.11561454061561581No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4570.11561454061561581No Hit
AAATCCCCGTCTGCAGAAGCGTGCGGCTACT4420.11181975263042053No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4330.10954287983930339No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG4330.10954287983930339No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4140.10473614839138937No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG3980.10068837454051442No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC3970.10043538867483474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)