FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007934714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387665
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT41061.059161905253247No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC39931.0300130267112069No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT31510.8128151883714031No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA28050.7235628700037403No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA20460.5277752698850812No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA18270.4712831955425432No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17540.4524525040950305No Hit
GTCTGGGCCATCTTCACCCGGGCGACGATGTACACGGTGACGGC15960.4116956650716469No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT15180.3915752002373183No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC14540.3750661008860743No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14280.36835927927463147No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT13590.3505604065365715No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT13410.34591722234403416No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA12950.33405130718532755No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11820.30490242864328737No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11560.2981956070318445No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT11540.2976796976771181No Hit
GTCTTCCGGGCCTTCCTCACCCGGGCGACGGTGCGCGCGGTGAC11250.29019901203358567No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC11210.28916719332413293No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG11200.28890923864676976No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11200.28890923864676976No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC10870.2803967342937846No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10700.2760115047786104No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9510.2453148981723911No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC8880.2290637534985103No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8650.223130795919157No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8460.21822965704925645No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8420.21719783833980372No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8270.21332851817935589No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8220.21203874479253995No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC7910.20404214979428112No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT7170.18495350366940527No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6920.1785046367353256No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC6890.17773077270323603No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6850.1766989539937833No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6770.1746353165748778No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT6740.17386145254278823No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6520.1681864496407981No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6450.1663807668992558No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG6190.15967394528781292No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6180.15941599061044973No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG6170.15915803593308656No Hit
TTCCTGACCTTCCTCACCTTCGTCGCCATCGCCACCTTCATCAC6110.15761030786890742No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG6050.15606257980472832No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6040.15580462512736512No Hit
GTCAGTGCCGAAGGACTGACGCAGCTTGGTGCGAACCTGGTTTG5880.15167735028955412No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5810.14987166754801182No Hit
GATTGCCGGAGATGGAGTTTCCGGACGCGCCGGAGATTCCCAGG5560.14342280061393214No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5540.14290689125920575No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5480.14135916319502662No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5420.1398114351308475No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG5400.13929552577612114No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC5170.13336256819676784No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5050.13026711206840957No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4930.12717165594005134No Hit
TTCCTGGCCCTCTTCGCCTTCATCACCATCGCCACCTTCGTCGC4820.12433415448905628No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC4800.12381824513432989No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT4710.1214966530380612No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG4690.12098074368333483No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG4610.11891710626442933No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4300.11092051126617053No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG4240.10937278320199142No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT4160.10730914578308591No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4140.10679323642835954No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT4060.10472959900945404No Hit
GTCAATGCACGCGCCAGAGTTGAAGGTCTGAGTAGCAGAGCTGC4020.10369778030000129No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG3980.10266596159054853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)