FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007934720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434045
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT52591.2116255226992592No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT43230.9959796795263164No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG28900.6658295798822703No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT27790.6402561946341969No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG25860.595790759022682No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25590.5895702058542317No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT23340.5377322627838127No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22430.5167666946975544No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22410.5163059129813728No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT17880.41193885426626276No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG15560.3584881751892085No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG15500.357105830040664No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15100.34789019571703395No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA13930.3209344653204161No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13060.30089046066652075No Hit
TGCGTCACGACGCTGCTGAATTTGCGGGTTGGTCTGCATTTTTT11500.26494948680436364No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10910.25135642617700926No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9680.2230183506318469No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9630.22186639634139316No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA9240.21288115287585388No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9070.20896450828831112No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG8430.19421949337050307No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8400.1935283207962308No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA8190.18869011277632503No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG7680.17694017901369674No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7530.17348431614233548No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7110.16380790010252394No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA7070.16288633667016092No Hit
CGGGATTTCTTCGTAGTCGTTTTCATACCAATCCGGGTTGTGCA7060.16265594581207019No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6800.15666578350171068No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC6360.14652858574571762No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6350.14629819488762685No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6240.1437638954486286No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6120.14099920515153958No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6050.13938646914490432No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5930.13662177884781532No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5860.13500904284118007No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT5650.13017083482127428No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5640.12994044396318355No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5290.12187676393000726No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5040.11611699247773848No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4930.11358269303874023No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG4930.11358269303874023No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA4920.11335230218064947No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4900.11289152046446797No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4490.10344549528274719No Hit
CAGCAGAGTACCACCTTTAGCGTCCATAATGATATCACCCTGGG4460.10275432270847494No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG4360.10045041412756742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)