FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434045
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58861.3560805907221603No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG52831.2171549032934372No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT43671.0061168772823095No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC31860.7340252738771326No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG30030.6918637468465251No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29480.6791922496515338No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG29380.6768883410706263No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG26530.6112269465147623No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA24310.5600801760186156No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG18910.43566911264961No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC17350.39972813878745295No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16560.38152726099828355No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15640.3603313020539345No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC14490.33383635337349815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13470.31033648584824153No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13130.302503196673156No Hit
GCTCGTCAGCGCTACCAATCCGCATCTCCGG12230.2817680194449884No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10320.237763365549655No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9840.22670460436129894No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9310.21449388888248916No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9140.21057724429494637No Hit
ATGTCCAACATGGACATCGATGGTATTAACA8540.19675379280950134No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8350.19237636650577705No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8200.1889205036344158No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7710.177631351587969No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7250.16703337211579444No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC7250.16703337211579444No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7100.16357750924443318No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA7090.16334711838634244No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7050.16242555495397942No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6790.15643539264361989No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6750.1555138292112569No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6670.15367070234653088No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6570.15136679376562337No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6390.14721975831998987No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6370.14675897660380838No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6150.14169037772581186No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6070.13984725086108582No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6060.13961686000299509No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5640.12994044396318355No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5590.1287884896727298No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5510.12694536280800378No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5050.11634738333582922No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5050.11634738333582922No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4830.11127878445783271No Hit
GCGCTGAAGGCGGGCGCAGTTGAAGCGGCGT4730.1089748758769252No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4630.10667096729601769No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4530.10436705871511018No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4390.10114158670183966No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG4370.10068080498565816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)