FastQCFastQC Report
Wed 31 May 2023
EGAF00007934722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406862
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT50411.2389950400873022No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT41661.023934405277465No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG27280.6704976134414126No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26380.6483770910038292No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG24230.5955336207362694No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23250.5714468296375675No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA20740.5097551503949742No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT20730.5095093668123344No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20280.4984491055935428No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT16100.3957115680501005No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG15110.37137899336875896No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14790.36351391872428485No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13950.3428680977825405No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13000.31951865743175817No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA12390.30452585889072953No Hit
TGCGTCACGACGCTGCTGAATTTGCGGGTTGGTCTGCATTTTTT11530.28338847078370555No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9700.23841007516061957No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9650.2371811572474205No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9020.22169679154111221No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA8930.2194847392973539No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8810.21653533630567615No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8510.20916182882648168No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA8490.20867026166120206No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG8370.20572085866952433No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7480.18384611981458085No Hit
CGGGATTTCTTCGTAGTCGTTTTCATACCAATCCGGGTTGTGCA6730.1654123511165948No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG6690.16442921678603556No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA6490.1595135451332393No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6360.1563183585589217No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6110.15017376899292634No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5820.14304604509637175No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5770.1418171271831727No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC5720.14058820926997362No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT5610.13788458986093566No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5470.13444361970397825No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5450.13395205253869863No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5400.13272313462549956No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5230.1285448137206227No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5200.1278074629727033No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5000.12289179131990699No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA4840.11895925399766998No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4660.11453514951015333No Hit
CAGCAGAGTACCACCTTTAGCGTCCATAATGATATCACCCTGGG4640.11404358234487369No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG4570.11232309726639499No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4490.11035682860527649No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG4330.10642429128303946No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA4250.10445802262192094No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4220.10372067187400151No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4080.1002797017170441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)