FastQCFastQC Report
Wed 31 May 2023
EGAF00007934723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406862
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52591.2925758611027818No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51971.2773372789791133No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG49731.222281756467795No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT41401.0175440321288298No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC29460.724078434456892No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27680.6803289567470051No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27110.6663192925365358No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25570.6284686208100043No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG25040.6154420909300943No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA21160.5200780608658464No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG16330.40136459045081624No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16130.39644891879801997No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC16110.3959573516327404No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14940.3672006724638821No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12990.3192728738491184No Hit
GCTCGTCAGCGCTACCAATCCGCATCTCCGG12100.29739813499417495No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11570.2843716051142648No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10210.2509450378752501No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9210.22636667961126866No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9040.22218835870639184No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8810.21653533630567615No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8280.203508806425766No Hit
ATGTCCAACATGGACATCGATGGTATTAACA8200.20154253776464748No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7740.19023649296321601No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC6860.16860753769091238No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6600.16221716454227722No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6460.15877619438531984No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6430.1580388436374004No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6270.15410630631516337No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6240.15336895556724392No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6230.15312317198460412No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6050.14869906749708747No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6050.14869906749708747No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6040.14845328391444765No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5990.14722436600124858No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5570.1369014555303764No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5520.13567253761717732No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5490.13493518686925787No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5450.13395205253869863No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5400.13272313462549956No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5380.13223156746021994No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4830.11871347041503015No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4830.11871347041503015No Hit
GCGCTGAAGGCGGGCGCAGTTGAAGCGGCGT4680.11502671667543295No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4650.1142893659275135No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4460.10961947785735704No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG4460.10961947785735704No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4300.10568694053512001No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4260.10470380620456075No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA4250.10445802262192094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)