FastQCFastQC Report
Wed 31 May 2023
EGAF00007934725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497947
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA46440.9326293762187542No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT35600.7149355252667453No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA20170.40506318945590597No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17030.342004269530693No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG16620.3337704615149805No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16390.32915149604275157No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG15470.31067563415383564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15190.3050525457528612No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC13950.2801502971199746No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG13340.2678999973892804No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13250.2660925761175386No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG12510.2512315567721063No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG11180.22452188686747787No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG9090.18254954844591895No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8150.1636720373855049No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC8030.16126214235651584No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7780.1562415277127887No Hit
AGCGAACAGGCAGCGGAAGCGATGGAAGTTG7660.15383163268379968No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7640.1534299835123015No Hit
TCTCAGAACTCCCGTGCCTCCATTGGCTCTA7280.1462002984253344No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6830.13716319206662556No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6560.13174092825140024No Hit
GCAGAAGCGATGGAAGTGGCGAACAAAACCC6500.13053598073690573No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6450.12953185780816032No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6370.12792526112216762No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG6340.12732278736492036No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6060.12169969896394597No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5720.11487166304847704No Hit
ATGTCCAACATGGACATCGATGGTATTAACA5620.11286341719098619No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5610.11266259260523712No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5520.11085517133349533No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5520.11085517133349533No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5360.10764197796150995No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5280.10603538127551729No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5240.10523208293252093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)