FastQCFastQC Report
Wed 31 May 2023
EGAF00007934784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587012
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26400.44973526946638226No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25220.42963346575538486No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT24290.41379051876281914No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA21000.3577439643482586No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA17100.2913057995407249No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16910.28806906843471686No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15680.2671154933800331No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC15150.258086717136958No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG13920.2371331420822743No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13350.22742294876425015No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG12830.21856452678991228No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11860.20204016272239747No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11820.20135874564744843No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11340.19318174074805966No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC9410.1603033668817673No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9410.1603033668817673No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9280.15808876138818287No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9160.15604451016333568No Hit
CAGAGAGGTGGCCTCGTTTTCCAGTTTCAGGTTGTACGGGTTAA8750.14905998514510777No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT8610.14667502538278604No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8590.1463343168453115No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8430.14360864854551525No Hit
TGGACGACCGCCGCCGCGACCACGACCGCGGCCACGACCGCGAC8230.14020156317076993No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7810.13304668388380475No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7700.13117278692769482No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7640.13015066131527125No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC7530.12827676435916133No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7410.1262325131343141No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6610.11260417163533283No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA6350.10817496064816391No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6120.1042568124672068No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6070.10340504112352046No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5940.10119043562993602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)