FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398524
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58621.4709277232989733No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA28090.7048508998203371No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17700.444138872439301No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT16000.4014814666117975No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15440.3874296152803846No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA13220.33172406178799774No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11870.2978490630426273No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA11130.27928054521183165No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10580.2654796197970511No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9720.243899990966667No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9710.2436490650500346No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT9240.2318555469683131No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8690.21805462155353256No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG7270.1824231413917355No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6730.16887314189358735No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6540.16410554947757222No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6470.16234906806114563No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6330.1588361052282924No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6190.15532314239543918No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5980.15005369814615932No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5940.14904999447962985No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5890.14779536489646797No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5780.14503517981351186No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5480.13750740231454067No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5180.12997962481556946No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5140.12897592114903994No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4890.12270277323323062No Hit
GCAGCTCACTCCCGTTCCGGCTCCGTGAGCC4620.11592777348415655No Hit
TCTCTGCCGCAGAAATCTCACGGTCGCACTC4520.11341851431783279No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA4440.11141110698477381No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC4410.11065832923487669No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4370.10965462556834721No Hit
CGCGCATCTATCGGTTCTAAAAGCGTCTCTA4270.10714536640202346No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4200.10538888498559686No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4180.10488703315233211No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4130.10363240356917024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)