FastQCFastQC Report
Wed 31 May 2023
EGAF00007934847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419385
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT77971.8591508995314567No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG28070.6693133993824291No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC26570.633546741061316No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG21620.5155167686016429No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG21350.5090787701038425No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG21260.5069327706045758No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC19750.4709276678946553No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17530.41799301357940793No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC17460.41632390285775595No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG17180.4096474599711482No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14710.3507516959357154No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC13560.32333059122286206No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12970.30926237228322423No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12690.3025859293966165No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12440.29662481967643095No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG12200.2909021543450529No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11740.27993371245991155No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11680.278503046127067No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG11100.2646732715762366No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG10910.26014282818889567No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10590.2525126077470582No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG10580.2522741633582508No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8990.21436150553787092No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC8900.21221550603860415No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8650.20625439631841866No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8320.19838573148777375No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG8300.19790884271015893No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8290.19767039832135153No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7950.1895632891018992No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7920.18884795593547696No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7900.18837106715786212No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7790.18574817888098047No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7400.1764488477174911No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7390.17621040332868365No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6930.16524196144354233No Hit
GAAGTATACATGAACGACCTGGGCACCGATA6850.16333440633308297No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6070.14473574400610417No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC5770.13758241234188157No Hit
CCGGAATCCTATTCCGGTGAACTGGAATGCA5750.13710552356426672No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5150.1227988602358215No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4940.11779152807086567No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC4850.11564552857159889No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG4510.1075384193521466No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4450.10610775301930207No Hit
CACACCGCATACGCGAACTCTATCGATGGCA4440.10586930863049465No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC4400.10491553107526497No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4390.10467708668645756No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4300.10253108718719078No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4200.10014664329911656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)