FastQCFastQC Report
Wed 31 May 2023
EGAF00007934852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453033
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT35250.778089013383131No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC29310.6469727370853778No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG25460.5619899654109082No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG21670.47833160056772905No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA20960.4626594530641256No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19890.4390408645727794No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG19750.4359505819664351No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT17870.3944525012526681No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA17480.3858438568492803No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16450.36310820624546114No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT15290.33750300750717943No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC15160.3346334593727168No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15050.3322053801820176No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13900.3068209159156176No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13400.29578419232153064No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT13350.29468051996212197No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12350.272607072773948No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT12080.26664724203314105No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC12050.26598503861749584No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC11440.2525202358327097No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11100.24501526378873062No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG10760.23751029174475147No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC10730.23684808832910628No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT10450.23066752311641758No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10400.22956385075700889No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG10140.22382475448808367No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA10050.221838144241148No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT9480.20925627934388885No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT9380.20704893462507148No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC9190.20285497965931842No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA9050.1997646970529741No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC8730.19270119395275842No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC8710.19225972500899494No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG8170.180340063527381No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7950.17548390514598275No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG7740.17084848123646623No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7650.16886187098953057No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7600.16775819863012187No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7540.16643379179883144No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT7500.16555085391130447No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG7490.16533011943942272No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA7370.16268130577684187No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC7280.1606946955299062No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC7270.16047396105802447No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG7150.1578251473954436No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7020.154955599260981No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6960.15363119242969056No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG6780.14965797193581926No Hit
TACCGGCAGCGGGCGGCCTGCGATATCCGCGTTGATCTTCAGCA6410.1414907964761949No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6290.13884198281361404No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6180.1364139036229149No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC6150.13575170020726968No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5540.12228689742248358No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5340.11787220798484879No Hit
ACCCTGGTGCAGACCCAGGACTGGAGACAGTTCCGGCATGGACG5260.11610633220979487No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG4970.10970503252522443No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG4930.10882209463769749No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4880.10771842227828878No Hit
TACCTGGATGTCCAGATCGAAGTAGAAACGGCGTGCCATGGCCG4880.10771842227828878No Hit
TTTGGAGGTGAACGGTTTACCTTTATCCGGCAGGTCTGCCATCG4870.10749768780640703No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG4820.10639401544699835No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT4810.1061732809751166No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4740.10462813967194443No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG4660.10286226389689052No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT4630.1022000604812453No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA4600.10153785706560006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)