FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453033
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39840.8794061359768494No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG36380.8030320087057676No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27730.6120966905280631No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG26140.5769999094988665No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG25520.5633143722421987No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25330.5591204172764456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23700.5231406983597221No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22520.4970940306776769No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22230.49069273099310645No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG21400.4723717698269221No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC20880.46089357728907165No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19860.4383786611571343No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC17900.39511470466831333No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16710.36884730251438635No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16280.35935572022347156No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC16080.3549410307858368No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG15450.3410347590572872No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13610.3004196162310472No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13420.29622566126529415No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG13160.29048656499636893No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT12480.27547662090841063No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11810.26068741129233414No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11190.24700187403566629No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11090.2447945293168489No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA11070.2443530603730854No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA10860.2397176364635689No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10760.23751029174475147No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10610.2341992746665254No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC10370.22890164734136365No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10140.22382475448808367No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10100.22294181660055667No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9950.21963079952233058No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9720.2145539066690506No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8940.1973366178622749No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8760.19336339736840363No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8260.18232667377431666No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC8150.17989859458361754No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC7950.17548390514598275No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG7950.17548390514598275No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7870.17371802937092884No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC7860.17349729489904708No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC7780.17173141912399317No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7770.17151068465211144No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG7770.17151068465211144No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7670.16930333993329405No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7620.16819966757388533No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7580.1673167296863584No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7380.1629020402487236No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6800.15009944087958274No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6570.1450225480263027No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6520.14391887566689404No Hit
TCCGTTTCTGAACCGAGCGAGTCCACCGGTC6220.13729684151044186No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6170.13619316915103313No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA6150.13575170020726968No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6060.133765089960334No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5980.1319992141852801No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5910.13045407288210792No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5130.11323678407533226No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5100.11257458065968705No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5030.11102943935651488No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC4990.11014650146898791No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG4910.10838062569393399No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4840.10683548439076182No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC4780.10551107755947138No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG4760.10506960861570791No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4730.1044074052000627No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG4720.10418667072818096No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA4710.10396593625629921No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC4630.1022000604812453No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4540.10021345023430964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)