FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464356
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC41540.8945722678289932No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG38250.823721455090491No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC28670.6174142252926634No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG26590.5726210062968929No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26060.5612073495335476No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG25880.5573310132742981No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23920.5151220184513605No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22590.4864802005357959No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC22250.4791582320461026No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG21670.46666781521074346No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG21030.45288528628896796No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC20190.43479571707913756No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16940.3648063123982462No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16640.35834575196616386No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC16500.35533082376452546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16360.3523158955628871No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG15230.32798111793537715No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13860.29847789196220137No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13610.29309409160213284No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG13400.28857169929967524No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12720.27392776232028876No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT11910.2564842491536666No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11490.24743946454875138No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11460.24679340850554315No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA11360.24463988836151573No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11130.23968679203025264No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC11120.2394714400158499No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA10870.23408763965578136No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC10800.23258017555496213No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC10420.2243967990076579No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10290.22159722282042224No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT10240.22052046274840856No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10160.21879764663318663No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9420.20286159756738364No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC8760.18864836461680262No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8640.1860641404439697No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8600.18520273238635873No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8570.1845566763431505No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8410.18111104411270665No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG8330.1793882279974847No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG8190.1763732997958463No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG8090.17421977965181887No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC8020.17271231555099967No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC7960.17142020346458323No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7760.16711316317652836No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7720.16625175511891738No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7690.16560569907570916No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG7530.16216006684526527No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7230.15569950641318298No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6910.14880824195229522No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6780.14600866576505955No Hit
TCCGTTTCTGAACCGAGCGAGTCCACCGGTC6730.14493190569304584No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6550.1410555694337965No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA6310.13588712108813067No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6010.12942656065604838No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5830.125550224396799No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5700.12275064820956336No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5590.12038177605113319No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC5400.11629008777748107No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5360.1154286797198701No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5320.11456727166225912No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA5310.11435191964785639No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC5200.11198304748942622No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5110.11004487935980153No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG4910.10573783907174668No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG4890.10530713504294119No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG4840.10423037497092748No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4740.10207685482690006No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4700.10121544676928909No Hit
ATTCTGAACGTCAGCGCAGTGGATAAGTCTA4690.10100009475488635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)