FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007934865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428817
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT48021.1198250069376914No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21930.5114069638097369No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC17900.41742748072021396No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC15900.3707875387403018No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14640.34140437529295714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14530.338839178484062No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14060.3278787921187826No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC13220.3082900164872195No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC11500.26817966638449503No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11030.2572192800192157No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10850.2530216852410236No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10060.23459890815895826No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9840.2294685145411679No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8950.20871374036010698No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG8850.2063817432611114No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8140.18982456385824256No Hit
CGCGATAACAACATTGAACGTGACAACAATA7590.1769985798137667No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7380.17210138590587595No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6670.15554420650300713No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6620.1543782079535093No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6330.14761541636642203No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6240.14551661897732598No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA6230.14528341926742644No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5930.1382874279704396No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5650.1317578360932519No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5620.13105823696355323No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5500.1282598404447585No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5410.12616104305566245No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5400.12592784334576287No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG5290.1233626465368677No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5140.1198646508883743No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5080.11846545262897692No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG5040.11753265378937867No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA4880.11380145843098571No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG4800.11193586075178923No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA4670.10890426452309493No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4660.10867106481319538No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4630.10797146568349669No Hit
CCGGGCATCGGCAAACCGGGCGGTCCGGGTC4610.10750506626369756No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4450.1037738709053046No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC4450.1037738709053046No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4440.10354067119540504No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC4410.10284107206570635No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4390.10237467264590722No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4380.10214147293600767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)