FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007934897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496045
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT75071.5133707627332198No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA15260.3076333800360854No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11640.2346561299882067No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11610.23405134614803091No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT10900.2197381285972039No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG10450.21066637099456703No Hit
TCTGGCTACGAAATTGACCACATCGACCTGG9240.18627342277414347No Hit
ACTCGTCTGGAAAACATCAAAACCGACCGTA9080.18304790895987258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8980.1810319628259533No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8910.1796208005322098No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC8850.1784112328518582No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8620.1737745567438438No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8350.1683315021822617No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA8350.1683315021822617No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8150.16429960991442308No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8040.16208206916711188No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7750.15623582537874586No Hit
GCTGAAGAGACTACCATCGAAAACGGCATCT7700.1552278523117862No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7460.1503895815903799No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7190.1449465270287978No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC6790.13688274249312057No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6470.1304317148645788No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6380.12861736334405144No Hit
ATGGAACGTCAGCTGCCAAAAAACGTTCGCC6110.12317430878246932No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5840.11773125422088722No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5820.11732806499410335No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5780.11652168654053563No Hit
TCTACCCAGCACACTGTTCAGTCCGGCGAAA5600.11289298349948089No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5550.11188501043252123No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5530.1114818212057374No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5510.11107863197895353No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5500.1108770373655616No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5480.11047384813877774No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5150.10382122589684403No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG4970.10019252285578929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)