FastQCFastQC Report
Sun 21 May 2023
EGAF00007934907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534702
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC66291.23975597622601No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC47320.8849789228392637No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG43400.8116670594087922No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA39660.7417215570542096No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC36540.683371298405467No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC34830.6513908681845214No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT32350.6050098933611618No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG30680.5737775433793029No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG26590.4972863389327139No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC26010.4864391754659605No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC25470.4763400922382935No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22600.4226653350838411No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20980.3923680854008401No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG20200.3777805207386544No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC17590.3289682851382639No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16700.31232349981859053No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG16570.3098922390415596No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC16230.30353355700932483No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT15910.2975489150966333No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15480.289507052526454No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14470.27061802648952127No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14240.2663165651147742No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG13780.2577136423652801No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13610.2545343013491627No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG12820.23975971662720544No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12520.2341491148340571No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC12100.22629427232364946No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC10710.2002984840153955No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10530.19693212293950652No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT10450.1954359624613336No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9960.18627197953252464No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC9330.17448971576691316No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT9170.1714973948105674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8920.1668218933162771No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG8890.16626083313696227No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8880.16607381307719066No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8670.1621463918219868No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC8460.158218970566783No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA8270.15466558943112238No Hit
CCGCCTGCGCCGCAGGCGCCGTACCAGGGCT8160.15260836877363465No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7700.14400544602414053No Hit
GGTGACGCGCGTGAAAACGACGTGTTCGAAC7410.13858186429076383No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6910.12923086130218328No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6640.12418131968834978No Hit
GTGGATATTAAAACCAAAGAAGCGTACGTTA6590.12324621938949172No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT6380.11931879813428788No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC6090.11389521640091116No Hit
CCACCGGCCCCGCAGGCTCCGTACCAGGGCT6060.11333415622159632No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6020.11258607598250989No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5980.11183799574342344No Hit
TCTCCGACCGAAAAAGCGGGTGCATCCGGCC5900.11034183526525056No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5730.10716249424913317No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5590.10454421341233061No Hit
GCCCAGTCTTCCGGCGATCGTCAGACCGTCT5570.1041701732927874No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5420.10136487239621322No Hit
TCTACCCAGCACACTGTTCAGTCCGGCGAAA5420.10136487239621322No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT5370.10042977209735517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)