FastQCFastQC Report
Sun 21 May 2023
EGAF00007934908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520714
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC44920.8626616530379441No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG44500.8545958049908394No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT40780.7831554365736277No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA35870.6888618320229531No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG33070.6350895117089228No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG31220.5995613715014384No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA26850.5156381430113268No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA25970.4987382709126315No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT23660.4543761066535565No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA23130.44419777459411497No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20300.38984932227672003No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG19590.3762141981970909No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT19560.3756380661937263No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC19040.3656517781354064No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG15890.30515791778212226No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT14440.27731153761949934No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT14380.27615927361277015No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT14130.27135817358473174No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13820.2654048095499641No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC13390.25714691750173796No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC12790.2456242774344458No Hit
GGCAATGCCGGCGCGCCAACGACCATCACGCGCTTTGTCAGTGA11570.22219490929761826No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11270.21643358926397216No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11020.21163248923593372No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9730.18685881309125546No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG9690.18609063708676932No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA9600.18436224107667548No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9580.1839781530744324No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG9100.17476004102059864No Hit
CGGTGCCTGTGGCGGAGGCGGTTCCTGATAGCCCTGGTATGGTG8960.17207142500489714No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC8950.1718793810037756No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC8290.15920447692975415No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC8270.15882038892751107No Hit
GTGCTGGGAACGCGGGGTCCAAGGGCCAGCGTAGCCCGGATAGC8160.15670790491517417No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC8050.15459542090283726No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7570.14537730884900346No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC7560.14518526484788194No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7320.14057620882096505No Hit
TGGGTCTTGAGACCACGGTGCCCAAGGGTGGCGATAGCACGGGT7310.14038416481984353No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7010.1346228447861974No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6830.13116605276600976No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG6760.12982174475815897No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6440.12367633672226981No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6430.12348429272114826No Hit
ATAAATACGGCTGCAGTCGATGTCCGGGCTAATGGTGTACGGCA6280.12060363270432523No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6130.11772297268750216No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA6080.11676275268189447No Hit
GTTCAGGCCGCGAATACCGAAGTTCATAGCCAGGTCGTGAGAAC6070.11657070868077293No Hit
CGGGATAGTGCCGTTGTCGCCGCCGGTCAGTTTGGTCAGCGGAA5970.11465026866955756No Hit
TTCCTGACCTTCCTCACCTTCGTCGCCATCGCCACCTTCATCAC5930.11388209266507143No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG5630.10812077263142532No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5580.10716055262581764No Hit
CGGGCAGGACAGACCAGAGGTCAGCTTACAGCGGCATACGTTTG5550.106584420622453No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5540.10639237662133148No Hit
ATCGTCGAACGGGATATACTGCACGAAGCCGTCGTAAGCGGTAA5470.10504806861348073No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5410.1038958046067515No Hit
CGGGTCCTGAGACCATGGCGCCCATGGGTGACGGTAGCAAGGGT5310.10197536459553612No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG5260.10101514458992844No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT5250.10082310058880692No Hit
CGGCAGTGGGTCCAGGAAGTGCAGTGGCTGCAGCTGCGGCTGTT5230.10043901258656385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)