FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520714
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC64141.2317702231935381No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC46610.8951170892274839No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45650.8766808651198162No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG40890.7852679205859646No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA39280.7543488364053972No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC34330.6592870558502364No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC32660.6272157076629398No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT29770.5717149913388155No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG29080.5584639552614296No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC25090.48183839881393625No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG24950.4791497827982347No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC23410.4495750066255181No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG23400.44938296262439653No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20040.3848561782475601No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG19110.3669960861432571No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC16570.31821690985838674No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15500.2976682017383823No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT15440.2965159377316531No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14790.28403307765875313No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14760.2834569456553886No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC14590.2801921976363224No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14040.2696297775746379No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13880.26655707355669334No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13310.25561056549276573No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG13240.25426625748491494No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG11920.22891644933687205No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC11540.22161877729425364No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10850.20836774121686763No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC10300.19780532115518307No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10100.19396444113275235No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT9800.18820312109910622No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9720.18666676909013394No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC9160.17591230502732785No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8940.17168733700265404No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8750.16803850098134485No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG8510.16342944495442796No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8020.15401928889947264No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC7830.15037045287816345No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA7540.14480117684563887No Hit
CCGCCTGCGCCGCAGGCGCCGTACCAGGGCT7520.1444170888433958No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7500.14403300084115273No Hit
GGTGACGCGCGTGAAAACGACGTGTTCGAAC7040.13519897678956203No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6870.13193422877049588No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6750.12962970075703745No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6480.12444451272675597No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG6140.1179150166886237No Hit
CCACCGGCCCCGCAGGCTCCGTACCAGGGCT5940.11407413666619295No Hit
GTGGATATTAAAACCAAAGAAGCGTACGTTA5920.11369004866394988No Hit
GCCCAGTCTTCCGGCGATCGTCAGACCGTCT5900.11330596066170681No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5800.11138552065049145No Hit
TCTACCCAGCACACTGTTCAGTCCGGCGAAA5740.11023325664376223No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5740.11023325664376223No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC5610.10773668462918223No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5560.10677646462357455No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT5410.1038958046067515No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5300.1017833205944146No Hit
TCTCCGACCGAAAAAGCGGGTGCATCCGGCC5230.10043901258656385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)