FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007934944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478850
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT28600.5972642790017751No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC25800.5387908530855173No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA20500.428109011172601No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA18530.38696877936723395No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA17000.3550172287772789No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG15400.3216038425394174No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14720.3074031533883262No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14500.3028088127806202No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG14190.29633496919703456No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14180.2961261355330479No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC13420.2802547770700637No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13270.2771222721102642No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA13130.2741986008144513No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12190.25456823639970766No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG11790.24621488984024223No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC11730.24496188785632245No Hit
CTGTGCGCGGTCGCCGCTACCCGGAGACACCAGACCGCCCGGCA10340.21593400856218023No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC10310.21530750757022032No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10090.21071316696251435No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT9560.19964498277122272No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9550.1994361491072361No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9190.19191813720371723No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC9030.1885767985799311No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8760.18293828965229195No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG7650.1597577529497755No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7330.1530750757022032No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6880.14367756082280464No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6830.14263339250287146No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6720.14033622219901848No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG6400.13365354495144616No Hit
GGCAACGTTGGTACAAACACGAGCGCCGTTACCGCTAGAGAAGC6370.13302704395948625No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6210.12968570533570012No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5760.12028819045630154No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5460.11402318053670253No Hit
ACCGCGAGACTGTGCGTTGTTCACCGCGTGGTGCGCGTCGGATT5250.10963767359298318No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT5090.10629633496919703No Hit
CGCCAAATTTAAATGGCCGCCTCATTTACGGCTGGCAGCGGAGG5090.10629633496919703No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5080.10608750130521041No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4980.10399916466534405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)