FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007934945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007934945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478850
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38440.8027566043646236No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT32600.6807977445964289No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG29210.6100031325049599No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23050.4813615954891929No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG21590.4508718805471442No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19450.4061814764540044No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17040.35585256343322547No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15300.319515505899551No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG15140.31617416727576486No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15140.31617416727576486No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14660.3061501514044064No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13910.2904876266054088No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG13690.2858932859977028No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG13370.27921060875013054No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG12430.25958024433538684No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11640.24308238488044273No Hit
CTGATGCGTATGAAGGAAGTATCTAGCGTTT11140.23264070168111098No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10790.22533152344157875No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG10310.21530750757022032No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10010.20904249765062127No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9990.208624830322648No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9900.2067453273467683No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9730.20319515505899552No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9180.19170930353973062No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG9070.18941213323587763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9020.18836796491594446No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8570.1789704500365459No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8540.17834394904458598No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7590.1585047509658557No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6890.14388639448679127No Hit
CTGTCCGACGAAGAGCGTGAAACCCTGGCAA6750.14096272319097838No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6620.13824788555915213No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5880.12279419442414118No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5820.12154119244022137No Hit
AAGGAATCCGTTGCAAAAAGCGAAGCTGGCG5810.12133235877623473No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5520.11527618252062233No Hit
TCCGAAAAATCTGAAAAATCTGACCGTAAAA5350.11172601023284955No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC5200.10859350527305002No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5120.10692283596115694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)