FastQCFastQC Report
Wed 31 May 2023
EGAF00007935030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427002
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA46841.0969503655720583No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA42220.9887541510344214No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20940.49039582952773053No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA17250.4039793724619557No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC12450.2915677209942811No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12330.2887574297075892No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11920.2791556011447253No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG11550.2704905363440921No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11210.2625280443651318No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10360.24262181441773106No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9920.23231741303319423No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9080.21264537402635114No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8790.20585383675017915No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8400.19672039006843062No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8360.19578362630619997No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG8280.19391009878173873No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8260.19344171690062342No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA7070.1655729949742624No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6600.15456602076805262No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6510.1524583023030337No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6100.14285647374016983No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6070.14215390091849686No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5920.13864103681013204No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5720.13395721799897894No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5520.12927339918782582No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT5490.12857082636615286No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5470.12810244448503755No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5300.1241211984955574No Hit
CTGTTTGTGGGTGGAAACCTGGTACAGGAAAGAGCCGCTGCGAC5050.118266424981616No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG4780.11194326958655931No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4540.10632268701317557No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4480.10491754136982964No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG4450.10421496854815669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)