FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435162
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT29520.6783680560343045No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT19560.4494877769658196No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC18420.42329063659051114No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15010.344929014941562No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC13130.3017267132700006No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12560.28862814308234636No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA11820.27162298178609345No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10380.23853185710149324No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA10110.23232727122313068No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9720.22336509162105148No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9260.2127943156801375No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8890.20429173503201106No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8420.19349115961412072No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8300.19073356589040402No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8090.18590777687389984No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8050.18498857896599427No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7240.16637482133090664No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7180.16499602446904832No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6940.15948083702161495No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG6760.1553444464360399No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6650.1528166521892996No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6590.15143785532744125No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6460.1484504621267482No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5950.1367306888009523No Hit
CGGGGTCAGGCGAGGCTGGGACAGCGGAGACAGAATGGTCGGCG5870.13489229298514116No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG5800.13328369664630643No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5690.13075590239956614No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5600.12868770710677863No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5530.1270791107679439No Hit
AGATGGCGCGTTGGAAGTAACTTTGTTGTTGGTGTCATCGGCGG5470.12570031390608555No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5440.1250109154751564No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5360.12317251965934525No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5080.11673813430400633No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4710.10823555365587988No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT4530.10409916307030487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)