FastQCFastQC Report
Sun 21 May 2023
EGAF00007935177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458119
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT68801.5017932022029212No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA61711.3470299201735794No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28780.628221051735466No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA28490.6218908187610642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27470.5996258614028233No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23290.508383193013169No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20810.4542487868872498No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17890.3905098893518933No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC17830.3892001859778791No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17660.3854893597515056No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17600.38417965637749146No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16560.3614781312279124No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC15610.34074116113935465No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC15610.34074116113935465No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14730.3215321783204801No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC14420.31476537755474016No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14420.31476537755474016No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13330.29097243292681596No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13150.2870433228047734No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12960.2828959287870619No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12500.2728548695862866No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12480.27241830179494847No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11260.2457876665233269No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10900.23792944627924187No Hit
ATGTCCAACATGGACATCGATGGTATTAACA10790.23552832342688254No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10100.22046673462571953No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9610.20977082373793707No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9270.2023491712851901No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8720.1903435570233935No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7650.16698718018680736No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7310.15956552773406038No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6980.1523621591769824No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6330.1381737059584955No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6210.13555429921046716No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA6180.13489944752346006No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6100.13315317635810783No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6020.1314069051927556No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5950.12987891792307238No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5810.12682294338370598No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5770.12594980780102988No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5340.1165636002872616No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT5270.11503561301757839No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5140.11219792237388101No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4970.10848709614750753No Hit
GGTCAGGTGAAACCGGAAGTTAAACCAACCC4960.1082688122518385No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4850.10586768939947917No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4790.10455798602546498No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4750.10368485044278887No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4700.10259343096444373No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG4660.10172029538176762No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4630.10106544369476055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)