FastQCFastQC Report
Sun 21 May 2023
EGAF00007935179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425733
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64911.5246645197811775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59551.3987640140651534No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA56711.332055537155917No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA26730.6278583055577087No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25410.5968529571351058No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22260.5228629211266216No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC19580.4599126682686096No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC16240.38145974119929627No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16120.37864107316087786No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16020.3762921831288625No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16020.3762921831288625No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15960.37488284910965325No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC13720.3222677123925089No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13640.3203886003668966No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC13630.3201537113636951No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13390.3145163752868582No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC13230.3107581512356336No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA11990.281631914838643No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11980.28139702583544146No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11390.2675385746465508No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11230.2637803505953262No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11180.26260590557931845No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10880.25555923548327236No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9900.23254011316952175No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9640.22643299908628178No Hit
ATGTCCAACATGGACATCGATGGTATTAACA9310.21868166198063108No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9070.21304432590379418No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8870.20834654583976342No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7790.18297853349399743No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7140.1677107482858975No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7070.16606652526348675No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6620.15549652011941756No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5830.13694028886649612No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5680.13341695381847307No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5490.12895406275764387No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5450.12801450674483772No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5360.1259005057160239No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5290.12425628269361313No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5230.1228469486744039No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4880.11462583356235011No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT4780.11227694353033474No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4780.11227694353033474No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4780.11227694353033474No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4730.11110249851432705No Hit
GGTCAGGTGAAACCGGAAGTTAAACCAACCC4560.10710938545990091No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4480.10523027343428862No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4440.10429071742148247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)