FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440047
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA44811.0183003179205858No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24540.5576677036771072No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21870.49699236672446356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21540.4894931677752604No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14770.33564596509009265No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9190.2088413283126575No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6530.14839323981301997No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6130.13930330169277375No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6030.13703081716271215No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5830.13248584810258904No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5550.12612289141841665No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5110.11612395948614579No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5100.11589671103313964No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5050.11476046876810887No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4940.11226073578504114No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4800.10907925744295496No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4570.10385254302381337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)