FastQCFastQC Report
Wed 31 May 2023
EGAF00007935356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526013
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT33860.6437103265508647No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG29060.5524578289890174No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG28330.5385798449848198No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG26090.49599534612262436No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC24520.4661481750451034No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT24070.45759325339868023No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG21700.4125373327275182No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG21280.4045527391908565No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT21260.4041725204510155No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG20070.38154950543047417No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG18060.3433375220764506No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17500.3326913973609017No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG15610.2967607264459243No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG15430.2933387577873551No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14590.2773695707140318No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA14060.26729377410824445No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13880.2638718054496752No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA13710.2606399461610264No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG13130.24961360270563654No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG13000.24714218089666984No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12470.23706638429088256No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10850.2062686663637591No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA10820.2056983382539975No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10500.19961483841654104No Hit
CACCTGACCATTGGCGTGAGTGGTAACGTTGTAGGCGTTAGTTT9900.18820827622131012No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9720.18478630756274084No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT9440.17946324520496643No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG8920.16957755796909962No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG8900.1691973392292586No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA8860.16843690174957654No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8790.16710613616013292No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG8560.16273362065196106No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8370.15912154262347128No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG8340.15855121451370974No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8300.15779077703402766No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG8190.155699573964902No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC8160.15512924585514046No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7990.1518973865664917No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG7550.14353257428998903No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7110.13516776201348635No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT7010.1332666683142812No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6880.13079524650531452No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6720.12775349658658627No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG6660.12661284036706316No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6550.1245216372979375No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6540.12433152792801698No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG6400.12166999674912977No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG6260.11900846557024256No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6250.11881835620032204No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5990.11387551258238865No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5850.11121398140350143No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG5810.11045354392381938No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5740.10912277833437577No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5640.10722168463517061No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT5640.10722168463517061No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5440.1034194972367603No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5440.1034194972367603No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5400.10265905975707826No Hit
GGAACGAGCGATGTTGCCATTTTTACGGCGGCGTTTCTTCGGCG5340.10151840353755516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)