FastQCFastQC Report
Sun 21 May 2023
EGAF00007935357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526013
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA54661.0391378159855365No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT34670.6591091855144264No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG31890.6062587806765232No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG31700.6026467026480334No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG31630.6013159370585899No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC31120.5916203591926436No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25770.4899118462851679No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG24270.46139544079709055No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23270.442384503805039No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA22150.4210922543739414No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG21790.4142483170568028No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG21140.40189120801196926No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20090.3819297241703152No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC18220.3463792719951788No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG18050.34314741270653004No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15070.2864948204702165No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG14640.27832011756363434No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14470.27508825827498556No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13610.25873885246182127No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG12410.23592572807135942No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11850.2252796033558106No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11490.21843566603867204No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11420.21710490044922845No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT11340.2155840254898643No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10300.19581265101813075No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9750.1853566356725024No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9570.1819346670139331No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9560.18174455764401262No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9180.17452040158703302No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG9180.17452040158703302No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9100.1729995266276689No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT9080.17261930788782787No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA8940.16995777670894066No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG8770.16672591742029189No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8750.16634569868045085No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8410.15988198010315335No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG8350.15874132388363024No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8330.15836110514378923No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7890.14999629286728655No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7540.14334246492006852No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7540.14334246492006852No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7480.1422018087005454No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7460.1418215899607044No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7410.1408710431111018No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG7400.14068093374118132No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7220.137258965082612No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT7200.136878746342771No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7090.13478754327364534No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6580.12509196540769907No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6530.12414141855809648No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG6440.12243043422881182No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6420.12205021548897081No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6420.12205021548897081No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6310.11995901241984513No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6100.11596671565151431No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6100.11596671565151431No Hit
GGTATGAACACCGCGAACACCGCTGGTACTT6030.1146359500620707No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5960.11330518447262711No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5960.11330518447262711No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5600.10646124715548856No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5520.10494037219612443No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5460.10379971597660134No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5430.10322938786683979No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5420.10303927849691927No Hit
TGCCCGGACTTCAAGCTGCGTCTGATGAAGG5360.10189862227739617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)