FastQCFastQC Report
Sun 21 May 2023
EGAF00007935433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438806
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59671.359826438107045No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA46241.053768635798052No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25210.5745135663596214No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21970.5006768366886506No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT21450.48882649735874173No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC17190.39174487130987273No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC15020.34229249372159903No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14890.33932990888912185No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14820.33773467090240333No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10990.25045236391480513No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10170.23176529035610272No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9350.21307821679740022No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9230.21034352310588278No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8700.19826529263501413No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8600.19598638122541623No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8080.18413604189550736No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA7340.16727209746448315No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7050.16066325437664936No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6800.1549659758526547No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6790.15473808471169492No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6770.15428230242977536No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6340.14448298336850454No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG6180.14083672511314796No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC6110.13924148712642945No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6000.1367346845758718No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5770.1314931883337967No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5740.13080951491091736No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5670.12921427692419885No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5650.1287584946422793No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5600.12761903893748033No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5500.12534012752788248No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5430.12374488954116397No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC5390.12283332497732484No Hit
CCGGCTGCCTCCCCGCCGCGTACCCCTGCCT5280.12032652242676717No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5270.12009863128580739No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5260.11987074014484761No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5190.1182755021581291No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5140.11713604645333017No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4920.11212244135221487No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC4870.11098298564741595No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4780.10893196537877788No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4640.10574148940534085No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4520.10300679571382343No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA4470.10186734000902449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)