FastQCFastQC Report
Wed 31 May 2023
EGAF00007935453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494665
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG26380.5332902065033912No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17150.34669928133181044No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG13790.2787745241729251No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13020.26320843399068056No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9250.18699523920228842No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC7910.15990619914487583No Hit
CGTTGGAGCCGTTCTCCGTCTGAAGGTCGCG6940.14029696865555477No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC6720.13584951431777062No Hit
GGCACTCTGGATCAGACCAACGGTCTGTTCG6310.12756107668826377No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6190.12513519250401786No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5660.11442087069026513No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA5400.10916478829106567No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4980.100674193646205No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA4960.10026987961549735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)