FastQCFastQC Report
Sun 21 May 2023
EGAF00007935465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493152
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA39900.8090811757835312No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT27090.5493235351372396No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19790.40129615209915No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18150.3680406852248394No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17930.36357958600999285No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17220.34918240218026086No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC17200.3487768477061839No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16330.3311352280838362No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15910.3226185841282201No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14320.29037700343910194No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13700.27780481474271623No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG13670.2771964830316008No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC13590.275574265135293No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG12540.2542826552462527No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12520.25387710077217573No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12350.25042988774252156No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11830.23988547141652067No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11750.23826325352021283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11450.23217993640905848No Hit
GCCGGTCAGCCGAGCGAACCGTGGCCGGATC11230.22771883719421193No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9680.19628836545324768No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9130.18513561741613133No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8840.17925507754201545No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG8130.16485789371228343No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7940.16100512620855234No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7910.16039679449743688No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7540.15289403672701318No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7170.14539127895658946No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7100.14397183829732008No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6800.1378885211861657No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6740.13667185776393484No Hit
ATGTCCAACATGGACATCGATGGTATTAACA6730.13646908052689638No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6280.12734410486016481No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6210.12592466420089546No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6130.12430244630458763No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5970.12105801051197197No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5870.11903023814158718No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5870.11903023814158718No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5850.11862468366751022No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5740.11639413406008695No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5520.1119330348452404No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5510.11173025760820192No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5310.10767471286743234No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5300.10747193563039388No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5190.10524138602297062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)