FastQCFastQC Report
Sun 21 May 2023
EGAF00007935565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414558
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA72301.7440261676291375No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA37800.9118145108766444No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA33020.7965109827816613No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT25930.6254854567997723No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25920.6252442360296991No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC24690.5955740813106971No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG24100.5813420558763792No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG24060.5803771727960865No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23470.5661451473617685No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC22980.5543253296281824No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG22890.5521543426975237No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC22660.5466062649858403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21460.517659772577058No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC18430.4445698792448825No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18110.43685081460254055No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13390.3229946111279966No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13070.31527554648565465No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12530.30224962490170254No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10810.2607596524491145No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10780.2600359901388949No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC10720.2585886655184558No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9870.23808490006223493No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9580.23108949773011253No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9490.22891851079945386No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT9150.22071700461696553No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8450.2038315507118425No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8370.20190178455125699No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA8120.19587126529942733No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG7970.19225295374832954No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7080.17078430521181595No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6870.16571866904027904No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6590.15896448747822983No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6370.1536576305366197No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6270.15124542283588785No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6050.14593856589427776No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC6050.14593856589427776No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5940.1432851374234727No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5680.13701339740156984No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5490.1324302027701793No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5440.13122409891981338No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC5430.1309828781497402No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC5310.12808822890886196No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5230.12615846274827647No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG5020.12109282657673957No Hit
AACACCCAGGAACGCATGGAGCTGCGTAAAC4940.11916306041615406No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4930.11892183964608087No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA4740.11433864501469033No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4710.11361498270447078No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4460.10758446345264112No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC4410.1063783596022752No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4240.10227760651103103No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4180.1008302818905919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)